Chronic wounds are all wounds that have a difficult healing process and a delay in physiological healing repair. Such wounds are considered a public health problem, which generate high costs for health services. The Baccharis dracunculifolia (BD) plant, popularly known as “alecrim do campo” or “vassourinha” and widely used in folk medicine, has several biological activities, such as antibacterial and immunomodulatory activities, which may favor the wound repair process. In this context, the objective of this study is to analyze the biological activity of Baccharis dracunculifolia extracts regarding cytotoxicity for subsequent production of a dressing with future applications in tissue repair of chronic wounds. FTIR and TG analyzes were performed to identify functional groups and biological cytotoxicity assays revealed that the ethanolic extract of Baccharis dracunculifolia (EEBD) showed lower cytotoxicity for most of the tested concentrations.