Disease such as megaesophagus and esophagitis does not have a cure. Esophagitis has treatments depending on it’s degree, while megaesophagus has a palliative treatment, which can be recommended euthanasia in cases of a poor prognosis, cases that the esophagus transplant could be at use, although it is not described in literature. In this perspective the regenerative medicine have techniques of a bioengineering of tissues, creating a minor immune response in transplants, as it removes the organ’s genetic material, preserving the extra cellular matrix (ECM). Different protocols have been evaluated for swine esophagus decellularization, using sodium dodecyl sulphate (SDS) in concentrations 0,5%, 1,0% and 1,5% for seven days, analysing the remaining EMC integrity. It has been concluded that the material in 0,5% of SDS showed more integrity of the remaining EMC compared to the other concentrations, demonstrating that it is the best biological scaffold for future medical applications.